Journal: Journal of Extracellular Vesicles

Article Title: Enhancing protective immunity against bacterial infection via coating nano‐ Rehmannia glutinosa polysaccharide with outer membrane vesicles

doi: 10.1002/jev2.12514

Figure Lengend Snippet: pRL‐OMV induced DC maturation in vitro. (a–c) Expression levels of (a) MHC II, (b) CD86 and (c) CD80 in BMDCs treated with pRL‐OMV, RL‐OMV, BL‐OMV, RGP‐OMV, OMV, LPS and medium for 24 h. (d) Quantitative data from the results of flow cytometry. Data are expressed in the form of mean ± SE ( n = 4). ns, not significant; * p < 0.05. (e) Surface topographical changes were documented by SEM after pRL‐OMV treatment. Scale bar, 5 µm.

Article Snippet: FITC‐conjugated anti‐mouse MHC Class II, FITC‐conjugated anti‐mouse CD80, PE‐conjugated anti‐mouse CD86, APC‐conjugated anti‐mouse CD11c, FITC‐conjugated anti‐mouse CD4, PE‐conjugated anti‐mouse CD8a, APC‐conjugated anti‐mouse CD3 and PE/Cy7‐conjugated anti‐mouse CD19 antibodies were all obtained from Multi Sciences (Hangzhou, Zhejiang, China).

Techniques: In Vitro, Expressing, Flow Cytometry

Journal: Journal of Extracellular Vesicles

Article Title: Enhancing protective immunity against bacterial infection via coating nano‐ Rehmannia glutinosa polysaccharide with outer membrane vesicles

doi: 10.1002/jev2.12514

Figure Lengend Snippet: pRL‐OMV increased the enrichment of antigen in inguinal lymph nodes and activated lymph node‐associated DCs. (a) Lymph node accumulation of OMVs labelled with OVA‐Cy5.5 in vivo ( n = 3). The inguinal draining lymph nodes, along with several organs, were collected 24 h after subcutaneous injection with the indicated OMV formulations to examine the accumulation of Cy5.5 fluorescence. (b) After ex vivo imaging, the inguinal lymph nodes were collected, frozen, and sectioned and stained for fluorescent imaging via CSLM. Cell nuclei are shown stained in blue (DAPI). Scale bar, 50 µm. (c) Expression of MHC I, MHC II, CD80 and CD86 on DCs in inguinal lymph nodes 7d following the second immunization ( n = 4). LNs were collected and processed into a single‐cell suspension. Following this, cells were analysed by flow cytometry. (d) Quantitative data derived from the flow cytometry results. Data are expressed in the form of mean ± SE ( n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: FITC‐conjugated anti‐mouse MHC Class II, FITC‐conjugated anti‐mouse CD80, PE‐conjugated anti‐mouse CD86, APC‐conjugated anti‐mouse CD11c, FITC‐conjugated anti‐mouse CD4, PE‐conjugated anti‐mouse CD8a, APC‐conjugated anti‐mouse CD3 and PE/Cy7‐conjugated anti‐mouse CD19 antibodies were all obtained from Multi Sciences (Hangzhou, Zhejiang, China).

Techniques: In Vivo, Injection, Fluorescence, Ex Vivo, Imaging, Staining, Expressing, Suspension, Flow Cytometry, Derivative Assay

Journal: Life sciences

Article Title: TPX2 influences the regulation of macrophage polarization via the NF-κB pathway in lung adenocarcinoma.

doi: 10.1016/j.lfs.2024.122437

Figure Lengend Snippet: Fig.2 Expression of TPX2 in A549 cells affects macrophage polarization. (A) Detection of macrophage surface markers CD80 and CD163 after cell supernatant induction in A549 group, Vehicle group and TPX2-shRNA group. (B) The analysis percentage of macrophage surface marker CD80, CD163; CD80/CD163 after induction by cell supernatant of the three groups. (C) CD80 and CD163 protein expression in macrophages after induction in the supernatant of the three groups cells. (D) The

Article Snippet: THP-1 cell differentiation into macrophages was induced by culturing for 24 h. The supernatant of each group was then applied for 48 h. The induced cells were digested and washed twice, followed by the addition of PE Anti-Human CD80 (B7-1) (PE-65083, Proteintech, China) and APC Anti-Human CD163 (GHI/61) (APC-65169, Proteintech, China) antibodies and resuspended to flow cytometry (BD Biosciences, USA).

Techniques: Expressing, shRNA, Marker

Journal: Acta Pharmaceutica Sinica. B

Article Title: Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy

doi: 10.1016/j.apsb.2023.11.032

Figure Lengend Snippet: The immune activation effect of OAP2 in vivo . (A–B) Analysis of mature DC cells in mouse spleen. Expression of CD80 and CD86 quantitatively detected by flow cytometry (A), and statistical analysis (B). (C–D) Analysis of mature DC cells in mouse tumor site. Expression of CD80 and CD86 quantitatively detected by flow cytometry (C), and statistical analysis (D). (E–F) Analysis of cytotoxic T cells in mouse tumor. Expression of CD4 and CD8 quantitatively detected by flow cytometry (E), and statistical analysis (F). (G–N) Tumor tissue cytokine expression content measured by ELISA ( n = 3).

Article Snippet: The following antibodies and kits were used in the flow cytometry and ELISA analyses: CoraLite® Plus 488 Anti-Mouse CD4, CoraLite®568 Anti-Mouse CD3, APC Anti-Mouse CD86, FITC Plus Anti-Mouse CD11c, and PE Anti-Mouse CD80 (B7-1) were purchased from Proteintech.

Techniques: Activation Assay, In Vivo, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Journal of neuroendocrinology

Article Title: GHR−/− Mice are Protected from Obesity-Related White Adipose Tissue Inflammation

doi: 10.1111/jne.12854

Figure Lengend Snippet: Flow Cytometry Antibodies (24 Months)

Article Snippet: Results were analyzed with FlowJo V10 software. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Target Conjugate Manufacturer Catalog # RRID CD206 AF488 BioRad MCA2235A488 AB_324891 Ly-6C PerCP-Cy5.5 Thermo Fisher 45–5932-80 AB_1518762 MHC-II Biotin Thermo Fisher 13–5321-81 AB_466661 F4/80 PE-Cy7 Thermo Fisher 25–4801-82 AB_469653 CD11c APC Thermo Fisher 117–0114-81 AB_469345 CD45 APC-eFluor 780 Thermo Fisher 47–0451-82 AB_1548781 CD11b Alexa Fluor 700 Thermo Fisher 56–0112-80 AB_657586 CCR2 Phycoerythrin R&D Systems FAB5538P AB_10718414 NK1.1 FITC Thermo Fisher 11–5941-81 AB_465317 NKT Phycoerythrin BD Biosciences 550082 AB_393552 CD44 Biotin Thermo Fisher 13–0441-81 AB_466441 CD4 PerCP-Cy5.5 Thermo Fisher 45–0042-80 AB_906231 CD3 PE-Cy7 Thermo Fisher 25–0031-81 AB_469571 CD25 APC Thermo Fisher 17–0251-81 AB_469365 CD62L Alexa Fluor 700 Thermo Fisher 56–0621-80 AB_494004 CD45 Alexa Fluor 700 Thermo Fisher 56–0451-80 AB_891456 CD16/CD32 Thermo Fisher 14–0161-82 AB_467133 Streptavidin eFluor 615 Thermo Fisher 42–4317-80 AB_11218079 Open in a separate window Flow Cytometry Antibodies (8 Months) table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Conjugate Manufacturer Catalog # RRID CD11b VioGreen Miltenyi Biotec 130–113-811 AB_2726328 CD11c APC-Vio770 Miltenyi Biotec 130–110-841 AB_2654715 CD206 APC BioLegend 141707 AB_10896057 CD25 PE Miltenyi Biotec 130–108-996 AB_2656655 CD3 APC-Vio770 Miltenyi Biotec 130–109-840 AB_2657087 CD38 PE-Vio770 Miltenyi Biotec 130–109-258 AB_2657842 CD4 VioGreen Miltenyi Biotec 130–109-413 AB_2657964 CD44 PE-Vio770 Miltenyi Biotec 130–110-085 AB_2658157 CD45 VioBlue Miltenyi Biotec 130–110-802 AB_2658222 CD62L PerCP-Vio700 Miltenyi Biotec 130–107-046 AB_2660523 CD80 PE Miltenyi Biotec 130–116-460 AB_2727557 CD8a FITC Miltenyi Biotec 130–102-490 AB_2659883 F4/80 PerCP-Vio700 Miltenyi Biotec 130–102-161 AB_2651711 GITR APC Miltenyi Biotec 130–116-427 AB_2727531 Open in a separate window Flow Cytometry Antibodies (24 Months)

Techniques: Flow Cytometry